Saccharomyces cerevisiae mutants that make mannoproteins with a truncated carbohydrate outer chain.
نویسندگان
چکیده
منابع مشابه
Glycolysis mutants in Saccharomyces cerevisiae.
Mutants have been isolated in S. cerevisiae with the phenotype of growth on pyruvate but not on glucose, or growth on rich medium with pyruvate but inhibition by glucose. Screening of mutagenized cultures was either without an enrichment step, or after enrichment using the antibiotic netropsin (Young et al. 1976) or inositol starvation (Henry, Donahue and Culbertson 1975). One class of mutants ...
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Aculeacin A is a lipopeptide that inhibits ,B-glucan synthesis in yeasts. A number of Saccharomyces cerevisiae mutants resistant to this antibiotic were isolated, and four loci (ACRI, ACR2, ACR3, and ACR4) whose products are involved in the sensitivity to aculeacin A of yeast ceils were defined. Mutants containing mutations in the four loci were also resistant to echinocandin B, another member ...
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Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Delta(9)-desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these were represented by respiratory-deficient (petite) strains. All strains were incapable of converting palmitate to palmitoleate and stearate to oleate whet...
متن کاملGlucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain.
Mannoproteins in the walls of mnn9 cells of Saccharomyces cerevisiae were released by laminarinase, and purified by concanavalin A affinity chromatography and ion-exchange chromatography. Carbohydrate analysis revealed that they contained N-acetylglucosamine, mannose, and glucose. An antiserum raised against beta (1-6)-glucan reacted with four proteins with molecular masses of 66, 100, 155, and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70728-5